姓名： 胡曉敏
職務/職稱： 教授
通訊地址： 中南民族大學生命科學學院8號樓8C303
E-mail：[email protected]
一,、研究領域和方向：
主要從事農(nóng)業(yè)和食品微生物的分子遺傳基礎與應用研究,。其中基礎研究包括芽胞桿菌和噬菌體的分子遺傳結構和進化,、基因調(diào)控,、代謝和功能蛋白的特性分析等,；應用研究包括芽胞桿菌殺蚊劑的研發(fā)和應用,，動物養(yǎng)殖業(yè)噬菌體/衍生酶抗菌劑的研發(fā)和應用,。
二,、教育背景：
2002.09 – 2007.03,，中國科學院武漢病毒研究所，微生物學專業(yè)，獲博士學位
2002.09-2003.09/2005.05-2005.10:丹麥奧胡斯大學國立環(huán)境調(diào)查研究所,，訪問學生
1996.09 – 2000.06,，華中師范大學，生物化學專業(yè),，獲學士學位
三,、工作簡歷：
2019.11–至今：中南民族大學
2007.03 – 2019.11：中國科學院武漢病毒研究所
2007/11-2009/09：比利時天主教魯汶大學
四、主持的主要科研項目情況：
（一）,、縱向項目
1.2022-2025,，國家自然科學基金面上項目“球形賴氨酸芽胞桿菌“整合可動員型”殺蚊基因島GI8與接合轉移質粒pBsph的互作”，負責人,。
2.2023-2024,，國家自然科學基金中比國際合作項目“攜殺蚊毒素基因的整合型接合轉移因子的鑒定和特性分析”，負責人,。
3.2022-2024,，武漢市科技知識創(chuàng)新項目“裂解酶表達文庫的構建及新型裂解酶抗菌劑的篩選和優(yōu)化”，負責人,。
4.2022-2024,，陜西省重點研發(fā)計劃“針對仔豬、犢牛和羔羊的功能性飼料添加劑產(chǎn)品和應用技術的研發(fā)與示范”,，負責人,。
5.2018-2020，國家科技重大專項“一帶一路”傳染病防控保障關鍵技術中“東非,、中亞和東南亞等區(qū)域重要傳染病防控技術”,，子課題負責人。
6.2017-2020,，農(nóng)業(yè)部兩減項目“棉花害蟲生防微生物流行病學”,，子課題科研骨干。
7.2016-2020,，“一帶一路”新發(fā)突發(fā)病原研究,、子課題科研骨干。
8.2016-2019,，國家自然科學基金面上項目“球形芽胞桿菌ICEBsph1的鑒定及對殺蚊毒素基因binA/binB的水平傳播分析”,，負責人。
9.2016-2018,， 國家重點研發(fā)項目“針對ZIKV,、DENV、CHIKV的傳播媒介伊蚊的防控產(chǎn)品及技術”,，子課題科研骨干,。
10.2013-2018,， 非洲病原微生物及流行病防控、子課題科研骨干,。
11.2012-2015,，國家自然科學基金面上項目“蠟狀芽胞桿菌群嘔吐毒素基因簇的水平轉移機理研究”，負責人,。
12.2009-2011,，國家自然科學基金青年基金項目“殺蚊球形芽胞桿菌的遺傳分型及毒素基因進化”，負責人,。
（二）,、橫向項目
1.2019-2021動物養(yǎng)殖常見病原菌的檢測和抗菌劑的研發(fā)、30萬,、負責人,。
2.2020-2021生物殺蚊幼制劑的研制和應用推廣、15萬,、負責人,。
五、第一作者和通訊作者論文（*通訊）：
1）Geng P, Zhao P, Wan X, Mahillon J, Hu Y, Gong Y,Hu X*.Interspecies horizontal transfer and specific integration of the mosquitocidal toxin-encoding plasmid pTAND672-2 fromBacillus thuringiensissubsp.israelensistoLysinibacillus sphaericus.Appl Environ Microbiol. 2023 Feb 28;89(2):e0165222.
2）Peiling Geng,Yunfei Gong,Xiaofu Wan,Xiaomin Hu*.Influence of the phagemid pfNC7401 on cereulide-producingBacillus cereusNC7401.Microorganisms. 2022 Apr 30;10(5):953.
3）Zhao P, Zhang M, Wan X, Geng P,Xiong H*,Hu X*. Characterization and heterologous expression of a novel Co(2+)-dependent leucyl aminopeptidase Amp0279 originating from Lysinibacillus sphaericus.Appl Microbiol Biotechnol. 2022 Feb;106(3):1139-1149.
4）Geng P, Cheng J, Yuan Z, Xiong H, Wang H,Hu X*.Horizontal transfer of large plasmid with type IV secretion system and mosquitocidal genomic island with excision and integration capability inLysinibacillus sphaericus.Environ. Microbiol.2021Sep;23(9):5131-5146.
5）Wan X,Geng P,Sun J,Yuan Z*,Hu X*.Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity againstBacillus cereusgroup strains.Virus Res.2021 Sep 16;198489.
6）Hu X*, Huang D, Ogalo J, Geng P, Yuan Z, Xiong H, Wan X, Sun J.Application ofBacillus thuringiensisstrains with conjugal and mobilizing capability drives gene transmissibility withinBacillus cereusgroup populations in confined habitats.BMC Microbiol.2020 Nov 26;20(1):363.
7）Zhang X, Karungu S, Cai Q, Yuan Z*,Hu X*.Effects of propoxur exposure on insecticidal susceptibility and developmental traits inCulex pipiens quinquefasciatus.Insects.10(9).288.2019. Sep
8）Karungu S, Atoni E, Ogalo J, Mwaliko C, Agwanda B,Yuan Z*,Hu X*.Mosquitoes of Etiological Concern in Kenya and Possible Control Strategies.Insects.10(6):173.2019. Jun
9）Tian S, Xiong H, Geng P,Yuan Z*,Hu X*.CesH Represses Cereulide Synthesis as an Alpha/Beta Fold Hydrolase inBacillus cereus.Toxins.11, 231.2019.
10）Fu P,Ge Y,Hu Y,Yuan Z*,Hu X*. A toxin-antitoxin system is essential for the stability of mosquitocidal plasmid pBsph ofLysinibacillussphaericus.Microbiol Res.2018 Sep; 214:114-122.
11）Geng P,Wan X,Cheng J,Yuan Z*,Hu X*.vB_LspM-01: A novel myovirus displaying pseudolysogeny inLysinibacillus sphaericusC3-41.Appl Microbiol Biotechnol.2018 Dec;102(24):10691-10702.
12）Hu Y,Cai Q,Tian S,Ge Y,Yuan Z*,Hu X*.Regulator DegU is required for multicellular behavior inLysinibacillus sphaericus.Res Microbiol.2018 Apr;169(3):177-187.
13）Fu P, Ge Y, Wu Y, Zhao N, Yuan Z*,Hu X*. The LspC3-41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3-41.BMC Microbiol.17(1):116.2017.
14）Fu P, Xiang X, Ge Y, Yuan Z,Hu X*. Differential expression of duplicated binary toxin genes binA/binB in Lysinibacillus sphaericus C3-41.Lett Appl Microbiol.65(1):90-97.2017.
15）Geng P, Hu Y, Zhou G, Yuan Z*,Hu X*. Characterization of three autolysins with activity against cereulide-producing Bacillus isolates in food matrices.Int J Food Microbiol.241:291-297.2017.
16）Xu K, Yuan Z, Rayner S*,Hu X*.Genome comparison provides molecular insights into the phylogeny of the reassigned new genusLysinibacillus.BMC Genomics.16:140.2015.
17）Shi T, Ge Y, Zheng D, Zhao N, Cai Q,Hu X*, Yuan Z*. Polyphosphate kinase of Lysinibacillus sphaericus and its effects on accumulation of polyphosphate and bacterial growth.Micriobiol. Res.172：41-7.2015.
18）Mei X, Xu K, Yang L, Yuan Z, Mahillon J,Hu X*. The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis.BMC Microbiol.14:149.2014.
19）Ge Y1,Hu X1, Zheng D, Wu Y, Yuan Z*.Allelic Diversity and Population Structure of Bacillus sphaericus Revealed by Multilocus Sequence Typing.Appl Environ Microbiol.77 (5): 5553–5556.2011.
20）Hu X, Swiecicka I, Timmery S, Mahillon J*. Sympatric soil communities ofBacillus cereus sensu lato: population structure and potential plasmid dynamics of pXO1- and pXO2-like elements.FEMS Microbiol. Ecology.70: 344-355.2009.
21）Hu X, Van der Auwera G, Timmery S, Zhu L, Mahillon J*. Distribution, diversity, and potential mobility of extrachromosomal elements related to theBacillus anthracispXO1 and pXO2 virulence plasmids.Appl Environ Microbiol.75: 3016-3028.2009.
22）HuX, Fan W, Han B, Liu H, Liu D, Zheng D, Li Q, Yan J, Berry C, Yuan Z*.Complete genome sequences of the mosquitocidal bacteriumBacillus sphaericusC3-41 and comparison with closely related Bacillus species.J Bacteriol.190: 2892-2902.2008.
23）Hu X, Li J, Hansen BM, Yuan Z*.Phylogenetic analysis and heterologous expressionofsurface layer protein SlpCofBacillus sphaericusC3-41.Biosci Biotech Bioch.72: 1257-1263.2008.
24）Hu X, Hansen BM, Hendriksen NB, Yuan Z*.Detection and phylogenic analysis of one anthrax virulence plasmid pXO1 conservative open reading frame ubiquitous presented withinBacillus cereusgroup strains.Biochem Biophysi Res Commu.349: 1214-1219.2006.
25）Hu X, Hansen BM, Johansen JE, Hendriksen NB, Smidt L, Jensen GB, Yuan Z*.Transfer and expression of the mosquitocidal plasmid pBtoxis inBacillus cereusgroup strains.FEMS Microbiol Lett.245: 239-247.2005.
26）Hu X, Hansen BM*, Eilenberg J, Hendriksen NB, Smidt L, Yuan Z, and Jensen GB. Conjugative transfer, stability and expression of a plasmid encoding acry1Ac gene inBacillus cereusgroup strains.FEMS Microbiol Lett.231: 45-52.2004.
27）胡曉敏;蔡亞君;周幗萍;袁志明*,。蠟狀芽胞桿菌群菌株中部分病原相關因子的檢測,。微生物學報, 2007, 47(3): 392~395。
28）張小磊,；袁志明,；胡曉敏*。致倦庫蚊對殺蟲劑的抗性現(xiàn)狀及治理.《中國媒介生物學及控制雜志》2019年30（06）：719-724,。
29）孫佳慧,、耿佩玲、萬小芙,、袁志明,、熊海容,，胡曉敏*,。噬菌體吸附及受體結合蛋白（RBP）的研究進展與應用?！渡锕こ虒W報》,。2021，37（8）：2614-22.
出版著作（*通訊）
30）Hu X,Mahillon J*. Chapter 5: Life cycle and gene exchange.Endospore-forming soil bacteria, Soil Biology, Edited by N. A Logan & P. De Vos. Spriger-Verlar Berlin Heidelberg, pp 89-114, Germany.2011.
31）胡曉敏,，袁志明*,。中國微生物基因組研究.主編喻子牛，科學出版社,。2012,。撰寫章節(jié)頁碼P307-331。
六、教學活動：
1.本科生理論課程：《生物信息學》,、《合成生物學》
2.本科生實驗課程：《分離技術模塊》
